PPARA Knockout cell line (HCT 116)

ENCN
location： Home > Products > KO Cell Lines > PPARA Knockout cell line (HCT 116)

PPARA Knockout cell line (HCT 116) Order

PPARA Knockout cell line (HCT 116)
Catalog#
YKO-H592
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PPARA Knockout cell line (HCT 116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PPARA Knockout cell line (HCT 116)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
PPARA
Gene ID
5465
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Accession
CVCL_D8TI(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 5 (based on transcript PPARA-202)
Validation: PCR+Sanger Sequencing
PPARA Knockout cell line (HCT 116) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HCT 116		 STR Info (Cell bank)
Cell Line:HCT 116
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 10121012
D7S820 11121112
D16S539 11131113
VWA 17221717
TH01 8989
AMEL XXXY
TPOX 8889
CSF1PO 710710
D12S391 1721
FGA 1823
D2S1338 1616
D21S11 2930
D18S51 1617
D8S1179 1214
D3S1358 1219
D6S1043 1313
PENTAE 1314
D19S433 1213
PENTAD 913
D1S1656 1214
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PPARA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PPARA gene knockout PPARA Knockout cell line (HCT 116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PPARA Knockout cell line (HCT 116)
PPARA Knockout cell line (HCT 116)
Catalog#
YKO-H592
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PPARA Knockout cell line (Hep G2)

    PPARA Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: PPARA
    Gene ID: 5465
    Size: 1*10^6
    Catalog#: YKO-HT1425
    YKO-HT1425(Hep G2)
  • Ppara Knockout cell line (Raw 264.7)

    Ppara Knockout cell line (Raw 264.7)
      inquiry   More details

    Organism: Mouse
    Gene Name: Ppara
    Gene ID: 19013
    Size: 1*10^6
    Catalog#: YKO-MT0141
    YKO-MT0141(Raw 264.7)
  • Ppara Knockout cell line (AML12)

    Ppara Knockout cell line (AML12)
      inquiry   More details

    Organism: Mouse
    Gene Name: Ppara
    Gene ID: 19013
    Size: 1*10^6
    Catalog#: YKO-M237
    YKO-M237(AML12)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PPARA Knockout cell line (Hep G2)

    PPARA Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: PPARA
    Gene ID: 5465
    Size: 1*10^6
    Catalog#: YKO-HT1425
      inquiry   More details
  • Ppara Knockout cell line (Raw 264.7)

    Ppara Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Ppara
    Gene ID: 19013
    Size: 1*10^6
    Catalog#: YKO-MT0141
      inquiry   More details
  • Ppara Knockout cell line (AML12)

    Ppara Knockout cell line (AML12)

    Organism: Mouse
    Gene Name: Ppara
    Gene ID: 19013
    Size: 1*10^6
    Catalog#: YKO-M237
      inquiry   More details
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now